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KMID : 0545120170270101820
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 10 p.1820 ~ p.1826
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Lipoteichoic Acid Isolated from Staphylococcus aureus Induces Both Epithelial-Mesenchymal Transition and Wound Healing in HaCaT Cells
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Kim Seong-Jae
Kim Hyeoung-Eun
Kang Bo-Yeon
Lee Youn-Woo
Kim Han-Geun
Chung Dae-Kyun
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Abstract
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Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, is recognized by Toll-like receptor 2, expressed on certain mammalian cell surfaces, initiating signaling cascades that include nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase. There are many structural and functional varieties of LTA, which vary according to the different species of gram-positive bacteria that produce them. In this study, we examined whether LTA isolated from Staphylococcus aureus (aLTA) affects the expression of junction proteins in keratinocytes. In HaCaT cells, tight junctionrelated gene expression was not affected by aLTA, whereas adherens junction-related gene expression was modified. High doses of aLTA induced the phosphorylation of extracellular signal-regulated protein kinases 1 and 2, which in turn induced the epithelial-mesenchymal transition (EMT) of HaCaT cells. When cells were given a low dose of aLTA, however, NF-κB was activated and the total cell population increased. Taken together, our study suggests that LTA from S. aureus infections in the skin may contribute both to the outbreak of EMTmediated carcinogenesis and to the genesis of wound healing in a dose-dependent manner.
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KEYWORD
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Lipoteichoic acid, Staphylococcus aureus, epithelial-mesenchymal transition, keratinocytes, Toll-like receptor, wound healing
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